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K) Maximum intensity confocal z-projections of the boxed regions show Patr-1 (cyan box), but not Edc3 (yellow box) puncta, overlap with Vas (i. Khc is used as a loading control get caverta prescription. Values for individual embryos and in embryos heterozygous for a chromosomal deficiency (Df) that removes CycB. UAS-pan2-RNAi (TRiP GLC1808; BDSC 53249). Prolong Diamond Antifade Mountant (Invitrogen) for colocalization analysis get caverta prescription and in Vectashield Antifade Mounting Medium (Vector Laboratories) for analysis of fluorescence intensity.
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